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Last updated: 2016/12/4
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PRKAA1 (phospho T172) polyclonal antibody

  • Catalog # : PAB12602
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKAA1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T172 of human PRKAA1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Dog, Human, Insect, Mouse, Rat
  • Specificity:
  • This antibody recognizes PRKAA1 with a phosphorylated site of Thr 172. It does not cross-react with non-phosphospecific peptide.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (BSA, 10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The whole cell lysate derived from serum starved HEK 293 was immunoblotted by PRKAA1 (phospho T172) polyclonal antibody (Cat # PAB12602) at 1 : 500 (lane 1). BSA was loaded as a negative control (Lane 2).
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5562
  • Gene Name:
  • PRKAA1
  • Gene Alias:
  • AMPK,AMPKa1,MGC33776,MGC57364
  • Gene Description:
  • protein kinase, AMP-activated, alpha 1 catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase, catalytic alpha-1 chain,AMP -activate kinase alpha 1 subunit,AMP-activated protein kinase, catalytic, alpha-1,AMPK alpha 1
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