PRKAA1 monoclonal antibody, clone 2B7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) against Jurkat (1), HeLa (2), HepG2 (3), MCF-7 (4), COS-7 (5), NIH/3T3 (6), K-562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and brain tissue (B) using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of PC-2 cells using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PRKAA1.
Immunogen
Recombinant protein corresponding to human PRKAA1.
Host
Mouse
Theoretical MW (kDa)
64
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) against Jurkat (1), HeLa (2), HepG2 (3), MCF-7 (4), COS-7 (5), NIH/3T3 (6), K-562 (7), HEK293 (8), and PC-12 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and brain tissue (B) using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PC-2 cells using PRKAA1 monoclonal antobody, clone 2B7 (Cat # MAB10302) (green) and negative control (purple). -
Gene Info — PRKAA1
Entrez GeneID
5562Gene Name
PRKAA1
Gene Alias
AMPK, AMPKa1, MGC33776, MGC57364
Gene Description
protein kinase, AMP-activated, alpha 1 catalytic subunit
Omim ID
602739Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-1 chain|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1
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Interactome
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Pathway
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Disease
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