PRKAA1 (phospho Ser496) recombinant monoclonal antibody, clone R01-3B7
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More Files
- More Functions
Western Blot
Western blot analysis of Ramos lysates with PRKAA1 (phospho Ser496) recombinant monoclonal antibody, clone R01-3B7 (Cat # RAB01795).
Immunocytochemistry
Immunocytochemical staining of HeLa with PRKAA1 (phospho Ser496) recombinant monoclonal antibody, clone R01-3B7 (Cat # RAB01795).(A) PRKAA1 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PRKAA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser496 of human PRKAA1.
Theoretical MW (kDa)
Calculated MW: 64 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Ramos lysates with PRKAA1 (phospho Ser496) recombinant monoclonal antibody, clone R01-3B7 (Cat # RAB01795).Immunocytochemistry
Immunocytochemical staining of HeLa with PRKAA1 (phospho Ser496) recombinant monoclonal antibody, clone R01-3B7 (Cat # RAB01795).(A) PRKAA1 (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — PRKAA1
Entrez GeneID
5562Protein Accession#
Q13131Gene Name
PRKAA1
Gene Alias
AMPK, AMPKa1, MGC33776, MGC57364
Gene Description
protein kinase, AMP-activated, alpha 1 catalytic subunit
Omim ID
602739Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-1 chain|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1
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Interactome
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Pathway
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Disease
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