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Last updated: 2017/7/16
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ATG5 polyclonal antibody

  • Catalog # : PAB12482
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ATG5.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1-50 of human ATG5.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275.
  • Form:
  • Liquid
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) using ATG5 polyclonal antibody (Cat # PAB12482). Lane 2 : ATG5 KO ES cell lysate (negative control). Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry
  • Immunohistochemistry
  • Staining of ATG5 in human liver hepatocytes using ATG5 polyclonal antibody (Cat # PAB12482) at 2.5 ug/mL .
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of ATG5 on SH-SY5Y cells using ATG5 polyclonal antibody (Cat # PAB12482) at 1 : 250. Photo courtesy of an anonymous collaborator.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Gene Information
  • Entrez GeneID:
  • 9474
  • Gene Name:
  • ATG5
  • Gene Alias:
  • APG5,APG5-LIKE,APG5L,ASP,hAPG5
  • Gene Description:
  • ATG5 autophagy related 5 homolog (S. cerevisiae)
  • Other Designations:
  • APG5 (autophagy 5, S. cerevisiae)-like,APG5 autophagy 5-like,ATG5 autophagy related 5-like,OTTHUMP00000017823,OTTHUMP00000017824,OTTHUMP00000040507,apoptosis specific protein
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