Rabbit polyclonal antibody raised against synthetic peptide of ATG5.
Immunogen:
A synthetic peptide corresponding to amino acids 1-50 of human ATG5.
Host:
Rabbit
Reactivity:
Human, Mouse
Specificity:
This antibody is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275.
Form:
Liquid
Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL) Western Blot (1:500) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (30% glycerol, 0.09% sodium azide)
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) using ATG5 polyclonal antibody (Cat # PAB12482). Lane 2 : ATG5 KO ES cell lysate (negative control). Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.
Staining of ATG5 in human liver hepatocytes using ATG5 polyclonal antibody (Cat # PAB12482) at 2.5 ug/mL .
Immunofluorescence
Immunofluorescent staining of ATG5 on SH-SY5Y cells using ATG5 polyclonal antibody (Cat # PAB12482) at 1 : 250. Photo courtesy of an anonymous collaborator.
APG5 (autophagy 5, S. cerevisiae)-like,APG5 autophagy 5-like,ATG5 autophagy related 5-like,OTTHUMP00000017823,OTTHUMP00000017824,OTTHUMP00000040507,apoptosis specific protein