ATG5 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Raw264.7 (A), K562 (B), C6 (C), Hela (D) whole cell lysates with ATG5 recombinant monoclonal antibody (Cat # RAB02469).
Immunofluorescence
Immunofluorescent analysis of MCF7 cells with ATG5 recombinant monoclonal antibody (Cat # RAB02469). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ATG5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human APG5L/ATG5.
Theoretical MW (kDa)
55
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of ATG5 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Raw264.7 (A), K562 (B), C6 (C), Hela (D) whole cell lysates with ATG5 recombinant monoclonal antibody (Cat # RAB02469).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of MCF7 cells with ATG5 recombinant monoclonal antibody (Cat # RAB02469). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — ATG5
Entrez GeneID
9474Protein Accession#
Q9H1Y0Gene Name
ATG5
Gene Alias
APG5, APG5-LIKE, APG5L, ASP, hAPG5
Gene Description
ATG5 autophagy related 5 homolog (S. cerevisiae)
Omim ID
604261Gene Ontology
HyperlinkGene Summary
S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
Other Designations
APG5 (autophagy 5, S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
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Interactome
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Pathway
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Disease
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