ATG5 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of ATG5 in rat spleen tissue lysate with ATG5 polyclonal antibody (Cat # PAB13023) at (A) 1 and (B) 2 ug/mL .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of ATG5 in human spleen tissue with ATG5 polyclonal antibody (Cat # PAB13023) at 2.5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG5.
Immunogen
A synthetic peptide corresponding to N-terminus 16 amino acids of human ATG5.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of ATG5 in rat spleen tissue lysate with ATG5 polyclonal antibody (Cat # PAB13023) at (A) 1 and (B) 2 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of ATG5 in human spleen tissue with ATG5 polyclonal antibody (Cat # PAB13023) at 2.5 ug/mL . -
Gene Info — ATG5
Entrez GeneID
9474Protein Accession#
EAW48415Gene Name
ATG5
Gene Alias
APG5, APG5-LIKE, APG5L, ASP, hAPG5
Gene Description
ATG5 autophagy related 5 homolog (S. cerevisiae)
Omim ID
604261Gene Ontology
HyperlinkGene Summary
S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
Other Designations
APG5 (autophagy 5, S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
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Interactome
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Pathway
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Disease
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Publication Reference
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The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy.
Hanada T, Noda NN, Satomi Y, Ichimura Y, Fujioka Y, Takao T, Inagaki F, Ohsumi Y.
The Journal of Biological Chemistry 2007 Dec; 282(52):37298.
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Autophagy as a cell death and tumor suppressor mechanism.
Gozuacik D, Kimchi A.
Oncogene 2004 Apr; 23(16):2891.
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Tor-mediated induction of autophagy via an Apg1 protein kinase complex.
Kamada Y, Funakoshi T, Shintani T, Nagano K, Ohsumi M, Ohsumi Y.
Journal of Cellular Biology 2000 Sep; 150(6):1507.
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The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy.
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