NOTCH1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOTCH1.
Immunogen
A synthetic peptide corresponding to amino acids 2488-2502 of human NOTCH1.
Sequence
CQHSYSSPVDNTPSHQ
Host
Rabbit
Reactivity
Human
Specificity
This antiserum is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:100000)
Western Blot (1:2000-1:10000)
Immunohistochemistry (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with NOTCH1 polyclonal antibody (Cat # PAB10292) was diluted 1 : 500 to detect NOTCH 1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOTCH1
Entrez GeneID
4851Protein Accession#
P46531 (human)Gene Name
NOTCH1
Gene Alias
TAN1, hN1
Gene Description
Notch homolog 1, translocation-associated (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq
Other Designations
OTTHUMP00000022594|neurogenic locus notch homolog protein 1|notch1|translocation-associated notch protein TAN-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells.
Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW.
Leukemia 2000 Dec; 14(12):2095.
Application:Flow Cyt, WB, Human Mouse , Leukemic B lineage cells.
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Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells.
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