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Western Blot (Transfected lysate)

Immunoblotting of NOTCH1 polyclonal antibody (Cat # PAB10291) was used at a 1 : 500 dilution to detect mouse NOTCH1 by Western blot.
Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods.
A reaction with diluted primary antibody was followed by washing, reaction with a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development.
Lane 1 : No transfection.
Lane 2 : N1 (mouse deleted extracellular domain)-myc.
Lane 3 : N1 (mouse intracellular domain)-myc.
Lane 4 : N2 (mouse deleted extracellular domain)-myc.
Lane 5 : N2 (mouse intracellular domain)-myc.
Lane 6 : N3 (mouse deleted extracellular domain)-myc.
Lane 7 : N3 (mouse intracellular domain)-myc.
Lane 8 : N4 (mouse deleted extracellular domain)-myc.
Lane 9 : N4 (mouse intracellular domain)-myc.
Lane 10 : N1 (mouse deleted extracellular domain) (V to G)-myc.
Personal communication, Dr. Stacey Huppert.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

4851

Protein Accession#

P46531 (human)

Gene Name

NOTCH1

Gene Alias

TAN1, hN1

Gene Description

Notch homolog 1, translocation-associated (Drosophila)

Omim ID

109730 190198

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq

Other Designations

OTTHUMP00000022594|neurogenic locus notch homolog protein 1|notch1|translocation-associated notch protein TAN-1