DCLRE1C polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Immunoblotting of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 500 dilution to detect DCLRE1C is various cell lysates by Western blot.
CJ is an DCLRE1C deficient cell lineand so no band is visible.
This antibody detects a band of approximately 90 KDa (predicted molecular weight: 78 KDa).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 1000 dilution to detect DCLRE1C by immunohistochemistry in human spleen.
Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DCLRE1C.
Immunogen
A synthetic peptide corresponding to amino acids 482-495 of human DCLRE1C.
Host
Goat
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:20000
Western Blot (1:500-1:2000)
Immunohistochemistry (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblotting of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 500 dilution to detect DCLRE1C is various cell lysates by Western blot.
CJ is an DCLRE1C deficient cell lineand so no band is visible.
This antibody detects a band of approximately 90 KDa (predicted molecular weight: 78 KDa).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 1000 dilution to detect DCLRE1C by immunohistochemistry in human spleen.
Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DCLRE1C
Entrez GeneID
64421Protein Accession#
Q96SD1;NP_071932Gene Name
DCLRE1C
Gene Alias
A-SCID, DCLREC1C, FLJ11360, FLJ36438, RS-SCID, SCIDA, SNM1C
Gene Description
DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq
Other Designations
OTTHUMP00000019166|OTTHUMP00000019167|OTTHUMP00000019168|OTTHUMP00000019170|OTTHUMP00000019171|OTTHUMP00000019172|OTTHUMP00000019174|OTTHUMP00000045150|artemis protein|severe combined immunodeficiency, type a (Athabascan)
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Interactome
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Pathway
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Disease
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Publication Reference
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Functional and biochemical dissection of the structure-specific nuclease ARTEMIS.
Pannicke U, Ma Y, Hopfner KP, Niewolik D, Lieber MR, Schwarz K.
The EMBO Journal 2004 May; 23(9):1987.
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Novel Artemis gene mutations of radiosensitive severe combined immunodeficiency in Japanese families.
Kobayashi N, Agematsu K, Sugita K, Sako M, Nonoyama S, Yachie A, Kumaki S, Tsuchiya S, Ochs HD, Sugita K, Fukushima Y, Komiyama A.
Human Genetics 2003 Apr; 112(4):348.
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Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-B-cell receptor checkpoint in bone marrow.
Noordzij JG, Verkaik NS, van der Burg M, van Veelen LR, de Bruin-Versteeg S, Wiegant W, Vossen JM, Weemaes CM, de Groot R, Zdzienicka MZ, van Gent DC, van Dongen JJ.
Blood 2003 Feb; 101(4):1446.
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Functional and biochemical dissection of the structure-specific nuclease ARTEMIS.
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