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DCLRE1C polyclonal antibody

  • Catalog # : PAB10023
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of DCLRE1C.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 482-495 of human DCLRE1C.
  • Host:
  • Goat
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:20000
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 500 dilution to detect DCLRE1C is various cell lysates by Western blot.
    CJ is an DCLRE1C deficient cell lineand so no band is visible.
    This antibody detects a band of approximately 90 KDa (predicted molecular weight: 78 KDa).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of DCLRE1C polyclonal antibody (Cat # PAB10023) was used at a 1 : 1000 dilution to detect DCLRE1C by immunohistochemistry in human spleen.
    Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q96SD1;NP_071932
  • Gene Name:
  • DCLRE1C
  • Gene Alias:
  • A-SCID,DCLREC1C,FLJ11360,FLJ36438,RS-SCID,SCIDA,SNM1C
  • Gene Description:
  • DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae)
  • Gene Summary:
  • This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019166,OTTHUMP00000019167,OTTHUMP00000019168,OTTHUMP00000019170,OTTHUMP00000019171,OTTHUMP00000019172,OTTHUMP00000019174,OTTHUMP00000045150,artemis protein,severe combined immunodeficiency, type a (Athabascan)
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