Western blot analysis of CASP9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 KDa pro-CASP9 protein. Cleaved CASP9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9 : recombinant human pro-CASP9 (full-length) protein. PRO : pro-CASP9. L : large subunit of cleaved CASP9. S : small subunit of cleaved CASP9.
Immunohistochemical analysis of CASP9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections. Using CASP9 polyclonal antibody (Cat # PAB0248) at 1 : 2000. Hematoxylin-eosin counterstain. A : Brain striatum. B : Brain stem motor neuron.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq