CASP9 recombinant monoclonal antibody, clone 2D5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HL-60 whole cell lysate and Lane 2: L02 whole cell lysate with CASP9 recombinant monoclonal antibody, clone 2D5 (Cat # RAB04329).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with CASP9 recombinant monoclonal antibody, clone 2D5 (Cat # RAB04329) (diluated at 1:60). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of K562 cells with CASP9 recombinant monoclonal antibody, clone 2D5 (Cat # RAB04329) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CASP9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CASP9.
Theoretical MW (kDa)
Calculated MW: 47, 3
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HL-60 whole cell lysate and Lane 2: L02 whole cell lysate with CASP9 recombinant monoclonal antibody, clone 2D5 (Cat # RAB04329).Immunofluorescence
Immunofluorescent staining of HepG2 cells with CASP9 recombinant monoclonal antibody, clone 2D5 (Cat # RAB04329) (diluated at 1:60). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K562 cells with CASP9 recombinant monoclonal antibody, clone 2D5 (Cat # RAB04329) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — CASP9
Entrez GeneID
842Protein Accession#
P55211Gene Name
CASP9
Gene Alias
APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6
Gene Description
caspase 9, apoptosis-related cysteine peptidase
Omim ID
602234Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 6|OTTHUMP00000002322|OTTHUMP00000002323|OTTHUMP00000044594|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cysteine protease
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Interactome
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Pathway
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Disease
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