CASP9 recombinant monoclonal antibody, clone 8G5
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More Files
- More Functions
Immunofluorescence
Immunofluorescence staining of HepG2 Cells with CASP9 recombinant monoclonal antibody, clone 8G5 at 1:7, counter-stained with DAPI.
Flow Cytometry
Overlay Peak curve showing HepG2 cells stained with CASP9 recombinant monoclonal antibody, clone 8G5 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CASP9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CASP9.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunofluorescence(1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescence staining of HepG2 Cells with CASP9 recombinant monoclonal antibody, clone 8G5 at 1:7, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing HepG2 cells stained with CASP9 recombinant monoclonal antibody, clone 8G5 (red line) at 1:50. -
Gene Info — CASP9
Entrez GeneID
842Protein Accession#
P55211Gene Name
CASP9
Gene Alias
APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6
Gene Description
caspase 9, apoptosis-related cysteine peptidase
Omim ID
602234Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 6|OTTHUMP00000002322|OTTHUMP00000002323|OTTHUMP00000044594|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cysteine protease
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Interactome
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Pathway
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Disease
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