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LAG3 (Human) Recombinant Protein BioActive

  • Catalog # : P6769
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  • Specification
  • Product Description:
  • Human LAG3 (NP_002277.4, 23 a.a. - 450 a.a.) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 60
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purification:
  • Ni-sepharose purification
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human LAG3 at 2 ug/mL (100 uL/well) can bind Anti-LAG3 antibody with a linear range of 13-40 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human FGL1 at 5 ug/mL (100 uL/well) can bind recombinant Human LAG3, the EC50 of Human LAG3 is 0.32 ug/mL.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6769
    LAG3 (Human) Recombinant Protein (Cat #P6769) was determined by SDS-PAGE with Coomassie Blue, showing a band at 60kDa.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4.
  • Storage Instruction:
  • Store the lyophilized protein at -20°C to -80°C for long term.
    After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 3902
  • Gene Name:
  • LAG3
  • Gene Alias:
  • CD223
  • Gene Description:
  • lymphocyte-activation gene 3
  • Gene Summary:
  • Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq
  • Other Designations:
  • lymphocyte-activation protein 3
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