LAG3 (Human) Recombinant Protein
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More Files
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human LAG3 (P18627, 23 a.a. - 450 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cell.
Sequence
LQPGAEVPVVWAQEGAPAQLPCSPTIPLQDLSLLRRAGVTWQHQPDSGPPAAAPGHPLAPGPHPAAPSSWGPRPRRYTVLSVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRAAVHLRDRALSCRLRLRLGQASMTASPPGSLRASDWVILNCSFSRPDRPASVHWFRNRGQGRVPVRESPHHHLAESFLFLPQVSPMDSGPWGCILTYRDGFNVSIMYNLTVLGLEPPTPLTVYAGAGSRVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCHIHLQEQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPVSGQERFVWSSLDTPSQRSFSGPWLEAQEAQLLSQPWQCQLYQGERLLGAAVYFTELSSPGAQRSGRAPGALPAGHL
Host
Mammals
Theoretical MW (kDa)
86
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by gel clotting method)
Activity
Immobilized FGL-1-His (LC13SE1012) at 2.0 ug/mL (100 uL/well) can bind LAG-3/CD223, hFc, Human with EC50 = 0.306 ug/mL when detected by Mouse Anti–Human IgG FC-HRP.
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/mL
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — LAG3
Entrez GeneID
3902Protein Accession#
P18627Gene Name
LAG3
Gene Alias
CD223
Gene Description
lymphocyte-activation gene 3
Omim ID
153337Gene Ontology
HyperlinkGene Summary
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq
Other Designations
lymphocyte-activation protein 3
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Interactome
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Disease
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