TGFA (Human) Recombinant Protein
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Specifications
Product Description
Human TGFA (P01135) recombinant protein expressed in E.Coli.
Sequence
VVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA
Host
Escherichia coli
Theoretical MW (kDa)
5.6
Form
Lyophilized
Purity
>= 95%
Endotoxin Level
<= 1 EUs/ug (Kinetic LAL) (50% confidence)
Activity
ED50 <= 2 ng/mL
3T3 cell proliferation
The values provided above are minimum expected values to pass internal requirements.Quality Control Testing
Reducing and Non-Reducing SDS PAGE
Conformation
Monomer
Storage Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Storage Instruction
Stored at -20°C to-80°C for 12 month.
After reconstitution with sterile water at 0.1 mg/mL, store at -20°C to -80°C for 3 months, store at 4°C for 1 month.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot
Functional Study
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Gene Info — TGFA
Entrez GeneID
7039Protein Accession#
P01135Gene Name
TGFA
Gene Alias
TFGA
Gene Description
transforming growth factor, alpha
Omim ID
190170Gene Ontology
HyperlinkGene Summary
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM
Other Designations
transforming growth factor-alpha
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Interactomes
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Pathways
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