TGFA (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human TGF-alpha, hFc Tag at 0.2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human TGF-alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human TGFA (P01135-1, Val40-Ala89) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Val40-Ala89
Host
Human
Theoretical MW (kDa)
32.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 6.0 ng/mL, messured by ELISA at 0.2 ug/mL. The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human TGF-alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human TGF-alpha, hFc Tag at 0.2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). -
Gene Info — TGFA
Entrez GeneID
7039Protein Accession#
P01135-1Gene Name
TGFA
Gene Alias
TFGA
Gene Description
transforming growth factor, alpha
Omim ID
190170Gene Ontology
HyperlinkGene Summary
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM
Other Designations
transforming growth factor-alpha
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Interactome
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Pathway
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Disease
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