TGFA (Human) Recombinant Protein
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Specification
Product Description
Human TGFA recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence
MVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA with polyhistidine tag at the C-terminus.
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 98% as determined by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Activity
ED50 < 0.2 ng/mL, Measured by the induction of 3T3 cells proliferation. The specific activity of recombinant human TGF alpha is > 5 x 106 IU/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of TGFA (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TGFA
Entrez GeneID
7039Gene Name
TGFA
Gene Alias
TFGA
Gene Description
transforming growth factor, alpha
Omim ID
190170Gene Ontology
HyperlinkGene Summary
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM
Other Designations
transforming growth factor-alpha
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Interactome
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