Human KITLG (181 a.a.) partial recombinant protein with His tag expressed in Barley grain (Hordeum vulgare). This protein is endotoxins-free and thus optimal to use in stem cell cultures.
Host:
Plants
Theoretical MW (kDa):
20.7
Reactivity:
Human
Form:
Lyophilized
Preparation Method:
Hordeum vulgare (barley) expression system
Purification:
Chromatography
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by LAL assay.
Activity:
Activity has been measured by cell-based proliferation assay using TF-1 cells. The ED50 is typically 2 - 10 ng/mL.
Storage Buffer:
Lyophilized from PBS, pH 7.2
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq