KITLG (Human) Recombinant Protein
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Specification
Regulatory Status
RUO grade
Product Description
Human KITLG recombinant protein with His tag at the C-terminus expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 19.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
The ED50 for this effect is < 5 ng/mL, measured by its ability to induce TF-1 cells proliferation. The specific activity of recombinant human SCF is > 5 x 105 IU/mg.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 200 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — KITLG
Entrez GeneID
4254Gene Name
KITLG
Gene Alias
DKFZp686F2250, KL-1, Kitl, MGF, SCF, SF, SHEP7
Gene Description
KIT ligand
Omim ID
184745Gene Ontology
HyperlinkGene Summary
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
mast cell growth factor|steel factor|stem cell factor
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