KITLG (Human) Recombinant Protein
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Specification
Product Description
Human KITLG (P21583, 26 a.a. - 189 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA
Host
Escherichia coli
Theoretical MW (kDa)
18
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <= 2.0 ng/mL, corresponding to a specific activity of >= 5 x 105 units/mg.
Storage Buffer
Lyophilized from PBS, pH 7.0
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — KITLG
Entrez GeneID
4254Protein Accession#
P21583Gene Name
KITLG
Gene Alias
DKFZp686F2250, KL-1, Kitl, MGF, SCF, SF, SHEP7
Gene Description
KIT ligand
Omim ID
184745Gene Ontology
HyperlinkGene Summary
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
mast cell growth factor|steel factor|stem cell factor
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Interactome
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