Human CSF1 (159 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Theoretical MW (kDa):
Barley grain (Hordeum vulgare) expression system
> 98% by SDS-PAGE
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Bioactivity is assayed by measuring the dose dependent effect of human MCSF on proliferation of M-NFS-60 cells. The ED50 lies typically in the range of 0.51-0.76 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Lyophilized from 5 mM TrisHCl, 80 mM Arginine, pH 8.0
Store at -20°C on dry atmosphere. After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq