CSF1 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human M-CSF at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human M-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CSF1 (P09603-1, Glu33-Asn190) partial recombinant protein expressed in HEK293 cells.
Sequence
Glu33-Asn190
Host
Human
Theoretical MW (kDa)
20.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 42.8 ng/mL, messured by ELISA at 0.5 ug/mL. The ED50 was 2.5 - 7.0 ng/mL, measured in a cell proliferation assay using RAW264.7 cells.The affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human M-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
Cell proliferation assay
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human M-CSF at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200). -
Gene Info — CSF1
Entrez GeneID
1435Protein Accession#
P09603-1Gene Name
CSF1
Gene Alias
MCSF, MGC31930
Gene Description
colony stimulating factor 1 (macrophage)
Omim ID
120420Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000013362|OTTHUMP00000013363|OTTHUMP00000013364|colony stimulating factor 1|macrophage colony stimulating factor
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Interactome
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Pathway
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Disease
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