CSF1 (Human) Recombinant Protein
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of CSF1 (Human) Recombinant Protein (Cat # P5818) using protein specific antibody.
Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CSF1 (159 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).
Host
Plants
Theoretical MW (kDa)
21
Form
Lyophilized
Preparation Method
Barley grain (Hordeum vulgare) expression system
Purification
Chromatography
Concentration
100 ug/mL
Purity
> 98% by SDS-PAGE
Endotoxin Level
Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
Activity
Bioactivity is assayed by measuring the dose dependent effect of human MCSF on proliferation of M-NFS-60 cells. The ED50 lies typically in the range of 0.51-0.76 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from 5 mM TrisHCl, 80 mM Arginine, pH 8.0
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Western Blot (Recombinant protein)
Western blot analysis of CSF1 (Human) Recombinant Protein (Cat # P5818) using protein specific antibody.Functional Study
SDS-PAGE
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Gene Info — CSF1
Entrez GeneID
1435Protein Accession#
BAD92189Gene Name
CSF1
Gene Alias
MCSF, MGC31930
Gene Description
colony stimulating factor 1 (macrophage)
Omim ID
120420Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000013362|OTTHUMP00000013363|OTTHUMP00000013364|colony stimulating factor 1|macrophage colony stimulating factor
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Interactome
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