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Last updated: 2016/12/4
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CD63 monoclonal antibody, clone MEM-259 (PerCP)

  • Catalog # : MAB6493
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native CD63.
  • Immunogen:
  • Native purified CD63 from HPB-ALL T cell line.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 40-60
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts with CD63 (LAMP-3), a 40-60 KDa tetraspan glycoproteinexpressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelialcells. Cell surface exposition of CD63 is usually activation-dependent.
  • Form:
  • Liquid
  • Conjugation:
  • PerCP
  • Isotype:
  • IgG1
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.2% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of CD63 in human HeLa cell line using CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of (A) human skin fibroblasts and (B) human primary fibroblasts with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green) after co-incubation of living cells with human Transferrin-Dyomics 547 (red) ; cell nuclei stained with DAPI (blue) .
  • Flow Cytometry
  • Application Image
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 967
  • Gene Name:
  • CD63
  • Gene Alias:
  • LAMP-3,ME491,MLA1,OMA81H,TSPAN30
  • Gene Description:
  • CD63 molecule
  • Gene Summary:
  • The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq
  • Other Designations:
  • CD63 antigen,CD63 antigen (melanoma 1 antigen),granulophysin,lysosome-associated membrane glycoprotein 3,melanoma 1 antigen,melanoma-associated antigen ME491,melanoma-associated antigen MLA1,ocular melanoma-associated antigen,tetraspanin-30
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