CD63 recombinant monoclonal antibody, clone 2E5
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More Files
- More Functions
Immunohistochemistry
Immunohistochemical staining of human pancreatic cancer with CD63 recombinant monoclonal antibody, clone 2E5 (Cat # RAB04361) (diluated at 1:155).
Immunohistochemistry
Immunohistochemical staining of human liver cancer with CD63 recombinant monoclonal antibody, clone 2E5 (Cat # RAB04361) (diluated at 1:155).
Immunofluorescence
Immunofluorescent staining of A549 cells with CD63 recombinant monoclonal antibody, clone 2E5 (Cat # RAB04361) (diluated at 1:51). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD63.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD63.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemical staining of human pancreatic cancer with CD63 recombinant monoclonal antibody, clone 2E5 (Cat # RAB04361) (diluated at 1:155).Immunohistochemistry
Immunohistochemical staining of human liver cancer with CD63 recombinant monoclonal antibody, clone 2E5 (Cat # RAB04361) (diluated at 1:155).Immunofluorescence
Immunofluorescent staining of A549 cells with CD63 recombinant monoclonal antibody, clone 2E5 (Cat # RAB04361) (diluated at 1:51). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD63
Entrez GeneID
967Protein Accession#
P08962Gene Name
CD63
Gene Alias
LAMP-3, ME491, MLA1, OMA81H, TSPAN30
Gene Description
CD63 molecule
Omim ID
155740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq
Other Designations
CD63 antigen|CD63 antigen (melanoma 1 antigen)|granulophysin|lysosome-associated membrane glycoprotein 3|melanoma 1 antigen|melanoma-associated antigen ME491|melanoma-associated antigen MLA1|ocular melanoma-associated antigen|tetraspanin-30
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Interactome
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Pathway
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