CD63 recombinant monoclonal antibody, clone NK-1-C3
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More Files
- More Functions
Western Blot
Western blot anaysis of K562 cell lysate with CD63 recombinant monoclonal antibody, clone NK-1-C3 (Cat # RAB03321).
Immunofluorescence
Immunofluorescent staining of A431 cells with CD63 recombinant monoclonal antibody, clone NK-1-C3 (Cat # RAB03321).
Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03321 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03321- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD63.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a plasma membrane preparation of the human melanoma MeWo cells.
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot anaysis of K562 cell lysate with CD63 recombinant monoclonal antibody, clone NK-1-C3 (Cat # RAB03321).Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of A431 cells with CD63 recombinant monoclonal antibody, clone NK-1-C3 (Cat # RAB03321).
Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03321 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03321- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Flow Cytometry
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Gene Info — CD63
Entrez GeneID
967Gene Name
CD63
Gene Alias
LAMP-3, ME491, MLA1, OMA81H, TSPAN30
Gene Description
CD63 molecule
Omim ID
155740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq
Other Designations
CD63 antigen|CD63 antigen (melanoma 1 antigen)|granulophysin|lysosome-associated membrane glycoprotein 3|melanoma 1 antigen|melanoma-associated antigen ME491|melanoma-associated antigen MLA1|ocular melanoma-associated antigen|tetraspanin-30
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Interactome
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Pathway
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