Mouse monoclonal antibody raised against synthetic peptide of C5AR1.
A synthetic peptide corresponding to N-terminus extracellular domain (met1-Asn31) of human C5AR1.
This antibody recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the Nterminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognize the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. Serotec recommend the use of the monoclonal antibody for functional studies. Cross reacts with human.
Flow Cytometry (10 ul of the suggested dilution (1:50-1:100) to label 5 x 105 cells in 100 ul) The optimal working dilution should be determined by the end user.
In PBS (0.09% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.