C5AR1 monoclonal antibody, clone S5/1
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of C5AR1.
Immunogen
A synthetic peptide corresponding to N-terminus extracellular domain (met1-Asn31) of human C5AR1.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the Nterminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognize the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. Serotec recommend the use of the monoclonal antibody for functional studies. Cross reacts with human.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Flow Cytometry (10 ul of the suggested dilution (1:50-1:100) to label 5 x 105 cells in 100 ul)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Flow Cytometry
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Gene Info — C5AR1
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Interactome
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Pathway
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Disease
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