C5AR1 recombinant monoclonal antibody, clone 32-G1
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More Files
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Immunofluorescence
Immunofluorescent staining of U937 cells with C5AR1 recombinant monoclonal antibody, clone 32-G1 (Cat # RAB03112).
Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version RAB03112 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03112- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of U937 cells with C5AR1 recombinant monoclonal antibody, clone 32-G1 (Cat#RAB03112).
U937 cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03112 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human C5AR1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic C5AR1 C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.
Reactivity
Human
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of U937 cells with C5AR1 recombinant monoclonal antibody, clone 32-G1 (Cat # RAB03112).
Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version RAB03112 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03112- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.Flow Cytometry
Flow cytometric analysis of U937 cells with C5AR1 recombinant monoclonal antibody, clone 32-G1 (Cat#RAB03112).
U937 cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03112 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — C5AR1
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Interactome
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Pathway
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Disease
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