C5AR1 recombinant monoclonal antibody, clone S5/1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human C5AR1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C5a-R; amino acids: 1-31 (N terminal).
Reactivity
Bovine, Domestic ferret, Human, Rabbit
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Blocking
ELISA
Flow Cytometry
Functional Assays
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of U937 cells with C5AR1 recombinant monoclonal antibody, clone S5/1 (Cat # RAB03114).
Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03114 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03114- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Functional Study
Blocking
Flow Cytometry
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Gene Info — C5AR1
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Interactome
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Pathway
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Disease
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