ZAP70 monoclonal antibody, clone ZAP-03
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line) (1, 3) and RAMOS (human Burkitt lymphoma cell line) (2, 4).
Lane 1, 2 : Immunostaining with ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664 ; 0.5 ug/mL).
Lane 3, 4 : Immunostaining with Isotype mouse IgG1 control.
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line).
Lane 1, 2, 3, 4 : Immunostaining with dilution range of ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664) ; 4 ug/mL (1), 2 ug/mL (2), 1 ug/mL (3), 0.5 ug/mL (4).
Lane 5 : Immunostaining with anti-ZAP70 comparative antibody.
Lane 6 : Immunostaining with Isotype mouse IgG1 control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ZAP70.
Immunogen
Recombinant His fusion protein corresponding to 160 amino acids of human ZAP70.
Host
Mouse
Theoretical MW (kDa)
70
Reactivity
Human
Specificity
This antibody reacts with ZAP70, a 70 KDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Form
Liquid
Isotype
IgG1
Recommend Usage
Flow Cytometry (2-5 ug/mL)
Western Blot (0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line) (1, 3) and RAMOS (human Burkitt lymphoma cell line) (2, 4).
Lane 1, 2 : Immunostaining with ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664 ; 0.5 ug/mL).
Lane 3, 4 : Immunostaining with Isotype mouse IgG1 control.Western Blot (Cell lysate)
Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line).
Lane 1, 2, 3, 4 : Immunostaining with dilution range of ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664) ; 4 ug/mL (1), 2 ug/mL (2), 1 ug/mL (3), 0.5 ug/mL (4).
Lane 5 : Immunostaining with anti-ZAP70 comparative antibody.
Lane 6 : Immunostaining with Isotype mouse IgG1 control.Immunocytochemistry
Flow Cytometry
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Gene Info — ZAP70
Entrez GeneID
7535Gene Name
ZAP70
Gene Alias
FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70
Gene Description
zeta-chain (TCR) associated protein kinase 70kDa
Omim ID
176947Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq
Other Designations
syk-related tyrosine kinase|zeta-chain (TCR) associated protein kinase (70 kD)|zeta-chain associated protein kinase 70kDa|zeta-chain associated protein kinase, 70kD
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Interactome
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Pathway
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Disease
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Publication Reference
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Detection of HLA-G by a specific sandwich ELISA using monoclonal antibodies G233 and 56B.
Van Lierop MJ, Wijnands F, Loke YW, Emmer PM, Lukassen HG, Braat DD, van der Meer A, Mosselman S, Joosten I.
Molecular Human Reproduction 2002 Aug; 8(8):776.
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A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells.
Rajagopalan S, Long EO.
The Journal of Experimental Medicine 1999 Apr; 189(7):1093.
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Detection of HLA-G by a specific sandwich ELISA using monoclonal antibodies G233 and 56B.
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