Mouse monoclonal antibody raised against partial recombinant ZAP70.
Recombinant His fusion protein corresponding to 160 amino acids of human ZAP70.
Theoretical MW (kDa):
This antibody reacts with ZAP70, a 70 KDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Flow Cytometry (2-5 ug/mL) Western Blot (0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq
syk-related tyrosine kinase,zeta-chain (TCR) associated protein kinase (70 kD),zeta-chain associated protein kinase 70kDa,zeta-chain associated protein kinase, 70kD