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ZAP70 monoclonal antibody, clone ZAP-03

  • Catalog # : MAB3664
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ZAP70.
  • Immunogen:
  • Recombinant His fusion protein corresponding to 160 amino acids of human ZAP70.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 70
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts with ZAP70, a 70 KDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (2-5 ug/mL)
    Western Blot (0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. Do not freeze.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line).
    Lane 1, 2, 3, 4 : Immunostaining with dilution range of ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664) ; 4 ug/mL (1), 2 ug/mL (2), 1 ug/mL (3), 0.5 ug/mL (4).
    Lane 5 : Immunostaining with anti-ZAP70 comparative antibody.
    Lane 6 : Immunostaining with Isotype mouse IgG1 control.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blotting analysis (reducing conditions) of HPB-ALL (human peripheral blood T cell leukemia cell line) (1, 3) and RAMOS (human Burkitt lymphoma cell line) (2, 4).
    Lane 1, 2 : Immunostaining with ZAP70 monoclonal antibody, clone ZAP-03 (Cat # MAB3664 ; 0.5 ug/mL).
    Lane 3, 4 : Immunostaining with Isotype mouse IgG1 control.
  • Immunocytochemistry
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 7535
  • Gene Name:
  • ZAP70
  • Gene Alias:
  • FLJ17670,FLJ17679,SRK,STD,TZK,ZAP-70
  • Gene Description:
  • zeta-chain (TCR) associated protein kinase 70kDa
  • Gene Summary:
  • This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • syk-related tyrosine kinase,zeta-chain (TCR) associated protein kinase (70 kD),zeta-chain associated protein kinase 70kDa,zeta-chain associated protein kinase, 70kD
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