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Last updated: 2017/6/18
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Cdkn2a monoclonal antibody, clone 5-C3-1

  • Catalog # : MAB2417
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  • Specification
  • Product Description:
  • Rat monoclonal antibody raised against synthetic peptide of Cdkn2a.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 54-75 of mouse Cdkn2a.
  • Host:
  • Rat
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody is specific to residues 62-75 of murine p19ARF.
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Immunohistochemistry (Frozen sections) (5 ug/mL)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of endogenous Cdkn2a using Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417). Total cell lysates (25 ug) from NIH/3T3 cells, which have deleted the Cdkn2a gene (lane 1) and from wild type MEF.
  • Immunohistochemistry (Frozen sections)
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of Cdkn2a on sections from a wild type mouse testis taken at P11 were probed with Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417) (green). Sections were stained with DAPI to reveal the position of cell nuclei (blue).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent detection of Cdkn2a using Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417). Wild type MEF at passage 5 (panels 1, 2) and NIH/3T3 cells, which have deleted the Cdkn2a gene (panels 3, 4).
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of Cdkn2a using Cdkn2a monoclonal antibody, clone 5-C3-1 (Cat # MAB2417). Total cell lysates (250 ug) from NIH/3T3 cells which have deleted the Cdkn2a gene (lane 5) and from NIH/3T3 cells expressing an HA-tagged version of Cdkn2a.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Gene Name:
  • Cdkn2a
  • Gene Alias:
  • ARF-INK4a,Arf,INK4a-ARF,Ink4a/Arf,MTS1,Pctr1,p16,p16(INK4a),p16INK4a,p19,p19ARF
  • Gene Description:
  • cyclin-dependent kinase inhibitor 2A
  • Other Designations:
  • OTTMUSP00000008143,cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4),cyclin-dependent kinase inhibitor protein,mitochondrial smARF
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