Mouse monoclonal antibody raised against Adalimumab. Target gene is TNF alpha.
Immunogen:
Adalimumab.
Host:
Mouse
Specificity:
The product is specific for Adalimumab. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Adalimumab monoclonal antibody, clone 3C2 (Biotin) (Cat # MAB23127).
Form:
Lyophilized
Purification:
Protein A purification
Isotype:
IgG2b, kappa
Recommend Usage:
Blocking ELISA (0.001-0.1 ug/mL) (Direct/Indirect/Inhibitory) Sandwich ELISA The optimal working dilution should be determined by the end user.The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction:
Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) binds with Adalimumab. Coating antigen: Adalimumab, 1 ug/mL. Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) dilution start from 1000 ng/mL. EC50 = 0.7748 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) and Anti-Adalimumab monoclonal antibody, clone 3C2 (Biotin) (Cat # MAB23127) as the capture and detection antibodies, respectively. The sensitivity of detecting Rituximab is about 300 pg/mL.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations:
APC1 protein,OTTHUMP00000029281,OTTHUMP00000037669,TNF superfamily, member 2,TNF, macrophage-derived,TNF, monocyte-derived,cachectin,tumor necrosis factor alpha