Anti-Adalimumab monoclonal antibody, clone 15C7
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Specification
Product Description
Mouse monoclonal antibody raised against Adalimumab.
Target gene is TNF alpha.Immunogen
Adalimumab.
Host
Mouse
Specificity
The product is specific for Adalimumab. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Adalimumab monoclonal antibody, clone 3C2 (Biotin) (Cat # MAB23127).
Form
Lyophilized
Purification
Protein A purification
Isotype
IgG2b, kappa
Recommend Usage
Blocking
ELISA (0.001-0.1 ug/mL) (Direct/Indirect/Inhibitory)
Sandwich ELISA
The optimal working dilution should be determined by the end user.The optimal working dilution should be determined by the end user.Inhibitory IC50
0.258 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) binds with Adalimumab. Coating antigen: Adalimumab, 1 ug/mL. Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) dilution start from 1000 ng/mL. EC50 = 0.7748 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Sandwich ELISA
Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) and Anti-Adalimumab monoclonal antibody, clone 3C2 (Biotin) (Cat # MAB23127) as the capture and detection antibodies, respectively. The sensitivity of detecting Rituximab is about 300 pg/mL.Blocking
Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) blocks Adalimumab binding with TNF-alpha. Coating antigen: Adalimumab, 0.5 ug/mL. Anti-Adalimumab monoclonal antibody, clone 15C7 (Cat # MAB23125) final concentration: 15 ng/mL. TNF-alpha dilution start from 50 ug/mL. IC50 = 258.3 ng/mL. -
Gene Info — TNF
Entrez GeneID
7124Gene Name
TNF
Gene Alias
DIF, TNF-alpha, TNFA, TNFSF2
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations
APC1 protein|OTTHUMP00000029281|OTTHUMP00000037669|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
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Interactome
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Pathway
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Disease
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