Rabbit monoclonal antibody raised against Pembrolizumab. Target gene is PD-1.
Immunogen:
Pembrolizumab.
Host:
Rabbit
Specificity:
The product is specific for Pembrolizumab. This antibody blocks Pembrolizumab binding with human PD-1 recombinant protein in ELISA. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) was labeled with biotin.
Form:
Lyophilized
Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
Blocking (10-50 ug/mL) ELISA (0.01-0.1 ug/mL (Direct/Inhibitory)) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction:
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ELISA binding of Anti-Pembrolizumab monoclonal antibody, clone 90G12 (Cat # MAB23116) with Pembrolizumab. Coating antigen: Pembrolizumab, 1 ug/mL. Anti Pembrolizumab antibody dilution start from 1000 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Blocking
Anti-Pembrolizumab monoclonal antibody, clone 90G12 (Cat # MAB23116) blocks Pembrolizumab binding with Human PD-1 recombinant protein. Coating antigen: Pembrolizumab, 1 ug/mL. PD-1-Fc-biotin final concentration: 20 ng/mL. Anti-Pembrolizumab antibody dilution start from 10 ug/mL.
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq