Anti-Pembrolizumab monoclonal antibody, clone 47E5
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Specification
Product Description
Rabbit monoclonal antibody raised against Pembrolizumab.
Target gene is PD-1.Immunogen
Pembrolizumab.
Host
Rabbit
Specificity
The antibody is recommended was labeled with biotin as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti Pembrolizumab monoclonal antibody, clone 94B9 (Cat # MAB23118).
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab.
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Blocking (1-10 ug/mL)
ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.009 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) with Pembrolizumab. Coating antigen: Pembrolizumab, 1 ug/mL. Anti-Pembrolizumab monoclonal antibody, clone 47E5 dilutions start from 1000 ng/mL. EC50= 3.618 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Pembrolizumab monoclonal antibody, clone 94B9 (Cat # MAB23118) and Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Pembrolizumab monoclonal antibody, clone 47E5 was labeled with Biotin. The sensitivity of detecting Pembrolizumab is up to 78 pg/mL.Blocking
Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) blocks Pembrolizumab binding with Human PD-1 protein. Coating antigen: PD-1 protein, 0.5 ug/mL Pembrolizumab-biotin final concentration: 20 ng/mL. Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) dilutions start from 1.56 ug/mL. IC50= 9.06 ng/mL. -
Gene Info — PDCD1
Entrez GeneID
5133Gene Name
PDCD1
Gene Alias
CD279, PD1, SLEB2, hPD-1, hPD-l
Gene Description
programmed cell death 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
Other Designations
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Interactome
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