Rabbit monoclonal antibody raised against Pembrolizumab.
Immunogen:
Pembrolizumab.
Host:
Rabbit
Specificity:
The antibody is recommended was labeled with biotin as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti Pembrolizumab monoclonal antibody, clone 94B9 (Cat # MAB23118).
Form:
Lyophilized
Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab.
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
Blocking (1-10 ug/mL) ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory)) Sandwich ELISA The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction:
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ELISA binding of Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) with Pembrolizumab. Coating antigen: Pembrolizumab, 1 ug/mL. Anti-Pembrolizumab monoclonal antibody, clone 47E5 dilutions start from 1000 ng/mL. EC50= 3.618 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
The Sandwich ELISA assay is developed by using Anti-Pembrolizumab monoclonal antibody, clone 94B9 (Cat # MAB23118) and Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Pembrolizumab monoclonal antibody, clone 47E5 was labeled with Biotin. The sensitivity of detecting Pembrolizumab is up to 78 pg/mL.