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Last updated: 2021/4/11

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Anti-Pembrolizumab monoclonal antibody, clone 47E5 

  • Catalog # : MAB23117
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Pembrolizumab.
  • Immunogen:
  • Pembrolizumab.
  • Host:
  • Rabbit
  • Specificity:
  • The antibody is recommended was labeled with biotin as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti Pembrolizumab monoclonal antibody, clone 94B9 (Cat # MAB23118).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Pembrolizumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Blocking (1-10 ug/mL)
    ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.009 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) with Pembrolizumab. Coating antigen: Pembrolizumab, 1 ug/mL. Anti-Pembrolizumab monoclonal antibody, clone 47E5 dilutions start from 1000 ng/mL. EC50= 3.618 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
  • Sandwich ELISA
  • Sandwich ELISA
  • The Sandwich ELISA assay is developed by using Anti-Pembrolizumab monoclonal antibody, clone 94B9 (Cat # MAB23118) and Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Pembrolizumab monoclonal antibody, clone 47E5 was labeled with Biotin. The sensitivity of detecting Pembrolizumab is up to 78 pg/mL.
  • Blocking
  • Blocking
  • Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) blocks Pembrolizumab binding with Human PD-1 protein. Coating antigen: PD-1 protein, 0.5 ug/mL Pembrolizumab-biotin final concentration: 20 ng/mL. Anti-Pembrolizumab monoclonal antibody, clone 47E5 (Cat # MAB23117) dilutions start from 1.56 ug/mL. IC50= 9.06 ng/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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