Anti-Nivolumab monoclonal antibody, clone 40F11 (Biotin)
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Nivolumab monoclonal antibody, clone 40F11 (Biotin) (Cat # MAB23100) with Nivolumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Nivolumab, 1 ug/mL. Anti-Nivolumab monoclonal antibody, clone 40F11 (Biotin) (Cat # MAB23100) dilutions start from 1000 ng/mL. EC50 = 14.30 ng/mL.
Sandwich ELISA
Standard curve of Nivolumab Sandwich ELISA. The Nivolumab Sandwich ELISA assay is developed by using Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098) and Anti-Nivolumab monoclonal antibody, clone 40F11 (Biotin) (Cat # MAB23100) as the capture and detection antibodies, respectively. The sensitivity of detecting Nivolumab is up to 78 pg/mL.
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Specification
Product Description
Rabbit monoclonal antibody raised against Nivolumab.
Target gene is PD-1.Immunogen
Nivolumab.
Host
Rabbit
Specificity
The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098).
Form
Lyophilized
Conjugation
Biotin
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Nivolumab.
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (ELISA capture: 0.01-0.1 ug/mL) (Direct/Indirect ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
N/A
Storage Buffer
Lyophilized from PBS, pH 7.4 (1% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Nivolumab monoclonal antibody, clone 40F11 (Biotin) (Cat # MAB23100) with Nivolumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Nivolumab, 1 ug/mL. Anti-Nivolumab monoclonal antibody, clone 40F11 (Biotin) (Cat # MAB23100) dilutions start from 1000 ng/mL. EC50 = 14.30 ng/mL.Sandwich ELISA
Standard curve of Nivolumab Sandwich ELISA. The Nivolumab Sandwich ELISA assay is developed by using Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098) and Anti-Nivolumab monoclonal antibody, clone 40F11 (Biotin) (Cat # MAB23100) as the capture and detection antibodies, respectively. The sensitivity of detecting Nivolumab is up to 78 pg/mL. -
Gene Info — PDCD1
Entrez GeneID
5133Gene Name
PDCD1
Gene Alias
CD279, PD1, SLEB2, hPD-1, hPD-l
Gene Description
programmed cell death 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
Other Designations
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Interactome
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