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Last updated: 2022/5/15

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Anti-Nivolumab monoclonal antibody, clone 33H4 

  • Catalog # : MAB23098
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Nivolumab.
    Target gene is PD-1.
  • Immunogen:
  • Nivolumab.
  • Host:
  • Rabbit
  • Specificity:
  • The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Nivolumab monoclonal antibody, clone 40F11 (Cat # MAB23099).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Nivolumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect ELISA)
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.484 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098) with Nivolumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Nivolumab, 1 ug/mL. Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098) dilutions start from 1000 ng/mL. EC50 = 22.48 ng/mL.
  • Sandwich ELISA
  • Sandwich ELISA
  • Standard curve of Nivolumab Sandwich ELISA. The Nivolumab Sandwich ELISA assay is developed by using Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098) and Anti-Nivolumab monoclonal antibody, clone 40F11 (Cat # MAB23099) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Nivolumab monoclonal antibody, clone 40F11 (Cat # MAB23099) was labeled with Biotin. The sensitivity of detecting Nivolumab is up to 78 pg/mL.
  • Blocking
  • Blocking
  • Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098) blocks Nivolumab binding with Human PD-1 recombinant protein (PD-1 Fc Chimera, Human). Coating antigen: Nivolumab, 1 ug/mL. PD-1-Fc-biotin final concentration: 10 ng/mL. Anti-Nivolumab monoclonal antibody, clone 33H4 (Cat # MAB23098) dilutions start from 10 ug/mL. IC50 = 0.48 ug/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5133
  • Gene Name:
  • PDCD1
  • Gene Alias:
  • CD279,PD1,SLEB2,hPD-1,hPD-l
  • Gene Description:
  • programmed cell death 1
  • Gene Summary:
  • This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
  • Other Designations:
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