Anti-Adalimumab monoclonal antibody, clone 134D5
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Adalimumab, 0.8 ug/mL. Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) dilution start from 1000 ng/mL. EC50 = 7.295 ng/mL.
Sandwich ELISA
Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) and Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit. In this ELISA assay, Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) was labeled with Biotin. The sensitivity is <2 ng/mL and the detection range is 0-33 ng/mL.
Blocking
Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) blocks Adalimumab binding with TNF-alpha. Coating antigen: Adalimumab, 0.5 ug/mL. Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) final concentration: 3.5 ng/mL. TNF-alpha dilution start from 50 ug/mL. IC50 = 353.1 ng/mL.
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Specification
Product Description
Rabbit monoclonal antibody raised against Adalimumab.
Target gene is TNF alpha.Immunogen
Adalimumab.
Host
Rabbit
Specificity
The product is specific for Adalimumab. This antibody blocks Adalimumab binding with TNF-alpha (TNF-alpha, Human) in ELISA. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073).
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Adalimumab.
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect/Inhibitory ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.353 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Adalimumab, 0.8 ug/mL. Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) dilution start from 1000 ng/mL. EC50 = 7.295 ng/mL.Sandwich ELISA
Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) and Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit. In this ELISA assay, Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) was labeled with Biotin. The sensitivity is <2 ng/mL and the detection range is 0-33 ng/mL.Blocking
Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) blocks Adalimumab binding with TNF-alpha. Coating antigen: Adalimumab, 0.5 ug/mL. Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) final concentration: 3.5 ng/mL. TNF-alpha dilution start from 50 ug/mL. IC50 = 353.1 ng/mL. -
Gene Info — TNF
Entrez GeneID
7124Gene Name
TNF
Gene Alias
DIF, TNF-alpha, TNFA, TNFSF2
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations
APC1 protein|OTTHUMP00000029281|OTTHUMP00000037669|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
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Interactome
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Pathway
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