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Last updated: 2021/4/11

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Anti-Adalimumab monoclonal antibody, clone 4E12 

  • Catalog # : MAB23073
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Adalimumab.
  • Immunogen:
  • Adalimumab.
  • Host:
  • Rabbit
  • Specificity:
  • The product is specific for Adalimumab. This antibody blocks Adalimumab binding with TNF-alpha (TNF-alpha, Human) in ELISA. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Adalimumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect/Inhibitory ELISA)
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.175 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA binding of Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Adalimumab, 1 ug/mL. Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) dilution start from 1250 ng/mL. EC50 = 6.283 ng/mL.
  • Sandwich ELISA
  • Sandwich ELISA
  • Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) and Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit. In this ELISA assay, Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) was labeled with Biotin. The sensitivity is <2 ng/mL and the detection range is 0-33 ng/mL.
  • Blocking
  • Blocking
  • Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) blocks Adalimumab binding with TNF-alpha. Coating antigen: Adalimumab, 0.5 ug/mL. Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) final concentration: 3.5 ng/mL. TNF-alpha dilution start from 50 ug/mL. IC50 = 175.4 ng/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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