Anti-Adalimumab monoclonal antibody, clone 4E12

Specificity:
The product is specific for Adalimumab. This antibody blocks Adalimumab binding with TNF-alpha (TNF-alpha, Human) in ELISA. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074).

Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Adalimumab.

Recommend Usage:
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect/Inhibitory ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.

Storage Instruction:
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.

Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Adalimumab, 1 ug/mL. Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) dilution start from 1250 ng/mL. EC₅₀ = 6.283 ng/mL.

Sandwich ELISA
Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) and Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit. In this ELISA assay, Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) was labeled with Biotin. The sensitivity is <2 ng/mL and the detection range is 0-33 ng/mL.

Blocking
Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) blocks Adalimumab binding with TNF-alpha. Coating antigen: Adalimumab, 0.5 ug/mL. Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) final concentration: 3.5 ng/mL. TNF-alpha dilution start from 50 ug/mL. IC₅₀ = 175.4 ng/mL.

Gene Summary:
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq

Other Designations:
APC1 protein,OTTHUMP00000029281,OTTHUMP00000037669,TNF superfamily, member 2,TNF, macrophage-derived,TNF, monocyte-derived,cachectin,tumor necrosis factor alpha