Rabbit monoclonal antibody raised against Adalimumab.
Immunogen:
Adalimumab.
Host:
Rabbit
Specificity:
The product is specific for Adalimumab. This antibody blocks Adalimumab binding with TNF-alpha (TNF-alpha, Human) in ELISA. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074).
Form:
Lyophilized
Preparation Method:
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Adalimumab.
Purification:
Protein A purification
Isotype:
IgG
Recommend Usage:
ELISA (ELISA detection: 0.01-0.1 ug/mL) (Direct/Indirect/Inhibitory ELISA) Sandwich ELISA The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction:
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt. After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ELISA binding of Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) with Adalimumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Adalimumab, 1 ug/mL. Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) dilution start from 1250 ng/mL. EC50 = 6.283 ng/mL.
Sandwich ELISA
Standard curve of Adalimumab Sandwich ELISA. The Adalimumab Sandwich ELISA assay is developed by using Anti-Adalimumab monoclonal antibody, clone 4E12 (Cat # MAB23073) and Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) as the capture and detection antibodies, respectively. These two antibodies could be used to development Sandwich ELISA Kit. In this ELISA assay, Anti-Adalimumab monoclonal antibody, clone 134D5 (Cat # MAB23074) was labeled with Biotin. The sensitivity is <2 ng/mL and the detection range is 0-33 ng/mL.