NDC80 monoclonal antibody, clone ACBI-14
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human NDC80.
Immunogen
A synthetic peptide corresponding to human NDC80.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat NDC80, in native form and recombinant. Superfamily members of NDC80 are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (cell lysate) analysis of Jurkat cell lysate. -
Gene Info — NDC80
Entrez GeneID
10403Protein Accession#
O14777Gene Name
NDC80
Gene Alias
HEC, HEC1, KNTC2, TID3, hsNDC80
Gene Description
NDC80 homolog, kinetochore complex component (S. cerevisiae)
Omim ID
607272Gene Ontology
HyperlinkGene Summary
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM
Other Designations
highly expressed in cancer, rich in leucine heptad repeats|kinetochore associated 2
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Interactome
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Disease
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