NDC80 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
NDC80 polyclonal antibody (Cat # PAB6236) staining (0.5 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NDC80.
Immunogen
A synthetic peptide corresponding to human NDC80.
Sequence
C-YEKKATLIKSSEE
Host
Goat
Theoretical MW (kDa)
73.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NDC80 polyclonal antibody (Cat # PAB6236) staining (0.5 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NDC80
Entrez GeneID
10403Protein Accession#
NP_006092Gene Name
NDC80
Gene Alias
HEC, HEC1, KNTC2, TID3, hsNDC80
Gene Description
NDC80 homolog, kinetochore complex component (S. cerevisiae)
Omim ID
607272Gene Ontology
HyperlinkGene Summary
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM
Other Designations
highly expressed in cancer, rich in leucine heptad repeats|kinetochore associated 2
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Interactome
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Disease
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Publication Reference
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HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis.
Chen Y, Riley DJ, Chen PL, Lee WH.
Molecular and Cellular Biology 1997 Jul; 17(10):6049.
Application：IF, IP, WB-Ce, Human, Mouse, 3T3-L1 cells, CV1 cells, Recombinant protein, Spleens, Testes, Thymus, T24, U-937 cells.
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HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis.
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