NEFM monoclonal antibody, clone AAGI-14
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human NEFM.
Immunogen
A synthetic peptide corresponding to human NEFM.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysate with NEFM monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — NEFM
Entrez GeneID
4741Protein Accession#
P07197Gene Name
NEFM
Gene Alias
NEF3, NF-M, NFM
Gene Description
neurofilament, medium polypeptide
Omim ID
162250Gene Ontology
HyperlinkGene Summary
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
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Interactome
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