NEFM polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
NEFM polyclonal antibody (Cat # PAB17252) (0.03 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NEFM.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human NEFM.
Sequence
C-QASHITVERKDYLK
Host
Goat
Theoretical MW (kDa)
102, 59.5
Reactivity
Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_005373.2; NP_001099011.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NEFM polyclonal antibody (Cat # PAB17252) (0.03 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NEFM
Entrez GeneID
4741Protein Accession#
NP_005373.2;NP_001099011.1Gene Name
NEFM
Gene Alias
NEF3, NF-M, NFM
Gene Description
neurofilament, medium polypeptide
Omim ID
162250Gene Ontology
HyperlinkGene Summary
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
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Interactome
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Pathway
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Disease
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Publication Reference
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Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.
Levy E, Liem RK, D'Eustachio P, Cowan NJ.
European Journal of Biochemistry 1987 Jul; 166(1):71.
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Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.
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