GUCY1A3 monoclonal antibody, clone 3G6B2
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GUCY1A3.
Immunogen
Recombinant protein corresponding to amino acids 22-214 of human GUCY1A3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
77.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell, (2) Jurkat cell with GUCY1A3 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) GUCY1A3-hIgGFc transfected HEK293 cell lysate with GUCY1A3 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with GUCY1A3 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HepG2 cells with GUCY1A3 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of GUCY1A3 monoclonal antibody, clone 3G6B2.Flow Cytometry
Flow cytometric analysis of HEK293 cells with GUCY1A3 monoclonal antibody (green) and negative control (purple). -
Gene Info — GUCY1A3
Entrez GeneID
2982Gene Name
GUCY1A3
Gene Alias
GC-SA3, GUC1A3, GUCA3, GUCSA3, GUCY1A1
Gene Description
guanylate cyclase 1, soluble, alpha 3
Omim ID
139396Gene Ontology
HyperlinkGene Summary
Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit, such as alpha-1 (GUCY1A3), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM
Other Designations
GC-S-alpha-1|soluble guanylate cyclase large subunit
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