SPI1 monoclonal antibody, clone 5C7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) SPI1-hIgGFc transfected HEK293 cell lysate with SPI1 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SPI1 monoclonal antibody, clone 5C7.
Flow Cytometry
Flow cytometric analysis of HeLa cells with SPI1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SPI1.
Immunogen
Recombinant protein corresponding to amino acids 124-271 of human SPI1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
42
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry
Immunohistochemistry
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) SPI1-hIgGFc transfected HEK293 cell lysate with SPI1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SPI1 monoclonal antibody, clone 5C7.Flow Cytometry
Flow cytometric analysis of HeLa cells with SPI1 monoclonal antibody (green) and negative control (red). -
Gene Info — SPI1
Entrez GeneID
6688Gene Name
SPI1
Gene Alias
OF, PU.1, SFPI1, SPI-1, SPI-A
Gene Description
spleen focus forming virus (SFFV) proviral integration oncogene spi1
Omim ID
165170Gene Ontology
HyperlinkGene Summary
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
31 kDa transforming protein|SPI-1 proto-oncogene|hematopoietic transcription factor PU.1
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Interactome
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Pathway
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Disease
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