SPI1 monoclonal antibody, clone AEIG-19
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of SPI1 in Daudi cell lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human SPI1.
Immunogen
A synthetic peptide corresponding to human SPI1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts with human SPI1, in native form and recombinant. Superfamily members of SPI1 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:60)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of SPI1 in Daudi cell lysate.Immunohistochemistry
Flow Cytometry
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Gene Info — SPI1
Entrez GeneID
6688Protein Accession#
P17947Gene Name
SPI1
Gene Alias
OF, PU.1, SFPI1, SPI-1, SPI-A
Gene Description
spleen focus forming virus (SFFV) proviral integration oncogene spi1
Omim ID
165170Gene Ontology
HyperlinkGene Summary
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
31 kDa transforming protein|SPI-1 proto-oncogene|hematopoietic transcription factor PU.1
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Interactome
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Pathway
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Disease
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