NR1I2 monoclonal antibody, clone 1D12G1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) NR1I2-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with NR1I2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NR1I2 monoclonal antibody, clone 1D12G1.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with NR1I2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NR1I2.
Immunogen
Recombinant protein corresponding to amino acid 1-142 of human NR1I2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
49.8kDa
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) NR1I2-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with NR1I2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of NR1I2 monoclonal antibody, clone 1D12G1.Flow Cytometry
Flow cytometric analysis of HepG2 cells with NR1I2 monoclonal antibody (green) and negative control (red). -
Gene Info — NR1I2
Entrez GeneID
8856Gene Name
NR1I2
Gene Alias
BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR
Gene Description
nuclear receptor subfamily 1, group I, member 2
Omim ID
603065Gene Ontology
HyperlinkGene Summary
This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq
Other Designations
orphan nuclear receptor PXR|steroid and xenobiotic receptor
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Interactome
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Disease
- Anemia
- Asthma
- Birth Weight
- Bone Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma
- Cardiovascular Diseases
- Cholangitis
+ View More Disease
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