JAK2 monoclonal antibody, clone 5C12C9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: JAK2-hIgGFc transfected HEK293 cell with JAK2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with JAK2 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JAK2 monoclonal antibody, clone 5C12C9.
Flow Cytometry
Flow cytometric analysis of Hela cells with JAK2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human JAK2.
Immunogen
Recombinant protein corresponding to amino acid 745-955 of human JAK2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
130.7
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: JAK2-hIgGFc transfected HEK293 cell with JAK2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded ovarian cancer tissues with JAK2 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of JAK2 monoclonal antibody, clone 5C12C9.Flow Cytometry
Flow cytometric analysis of Hela cells with JAK2 monoclonal antibody (green) and negative control (red). -
Gene Info — JAK2
Entrez GeneID
3717Gene Name
JAK2
Gene Alias
JTK10
Gene Description
Janus kinase 2 (a protein tyrosine kinase)
Gene Ontology
HyperlinkGene Summary
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq
Other Designations
Janus kinase 2|OTTHUMP00000021024|OTTHUMP00000043260|tyrosine-protein kinase JAK2
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Interactome
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Pathway
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Disease
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