JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Pervanadate), Lane 2: Hela whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with Pervanadate) and Lane 4: A549 whole cell lysate (not treated) with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262).
Immunohistochemistry
Immunohistochemical staining of human ovarian cancer with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human cervical cancer with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262) (diluated at 1:100).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262).
Lane 1: rabbit control IgG, Lane 2: RAB04262 precipitates and Lane 3 : Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human JAK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Y1007/Y1008 of human JAK2.
Theoretical MW (kDa)
Calculated MW: 120 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Pervanadate), Lane 2: Hela whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with Pervanadate) and Lane 4: A549 whole cell lysate (not treated) with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262).Immunohistochemistry
Immunohistochemical staining of human ovarian cancer with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human cervical cancer with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262) (diluated at 1:100).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with JAK2 (phospho Y1007/Y1008) recombinant monoclonal antibody, clone 1A4 (Cat # RAB04262).
Lane 1: rabbit control IgG, Lane 2: RAB04262 precipitates and Lane 3 : Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — JAK2
Entrez GeneID
3717Protein Accession#
O60674Gene Name
JAK2
Gene Alias
JTK10
Gene Description
Janus kinase 2 (a protein tyrosine kinase)
Gene Ontology
HyperlinkGene Summary
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq
Other Designations
Janus kinase 2|OTTHUMP00000021024|OTTHUMP00000043260|tyrosine-protein kinase JAK2
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Interactome
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Pathway
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Disease
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