PKN1 monoclonal antibody, clone 4H10B1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PKN1-hIgGFc transfected HEK293 cell with PKN1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with PKN1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with PKN1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PKN1 monoclonal antibody, clone 4H10B1.
Flow Cytometry
Flow cytometric analysis of Hela cells with PKN1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PKN1.
Immunogen
Recombinant protein corresponding to amino acid 442-620 of human PKN1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
104
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PKN1-hIgGFc transfected HEK293 cell with PKN1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with PKN1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with PKN1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PKN1 monoclonal antibody, clone 4H10B1.Flow Cytometry
Flow cytometric analysis of Hela cells with PKN1 monoclonal antibody (green) and negative control (red). -
Gene Info — PKN1
Entrez GeneID
5585Gene Name
PKN1
Gene Alias
DBK, MGC46204, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1
Gene Description
protein kinase N1
Omim ID
601032Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
protein kinase C-like 1|protein kinase C-like PKN|protein kinase C-related kinase 1|serine-threonine kinase N|serine/threonine protein kinase N
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Interactome
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Disease
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