PKN1 monoclonal antibody (M01), clone 1B10
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.
Lane 1: PKN1 transfected lysate(103.9 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PKN1 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PKN1 is approximately 3ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi ( Cat # H00005585-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKN1 monoclonal antibody (M01) clone 1B10 (Cat # H00005585-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PKN1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (42.57 KDa) .
-
Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PKN1.
Immunogen
PKN1 (AAH40061, 462 a.a. ~ 615 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (42.57 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Transfected lysate)
Western Blot analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.
Lane 1: PKN1 transfected lysate(103.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PKN1 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PKN1 is approximately 3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi ( Cat # H00005585-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKN1 monoclonal antibody (M01) clone 1B10 (Cat # H00005585-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to PKN1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PKN1
Entrez GeneID
5585GeneBank Accession#
BC040061Protein Accession#
AAH40061Gene Name
PKN1
Gene Alias
DBK, MGC46204, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1
Gene Description
protein kinase N1
Omim ID
601032Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
protein kinase C-like 1|protein kinase C-like PKN|protein kinase C-related kinase 1|serine-threonine kinase N|serine/threonine protein kinase N
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com